Recombinant human NT5E (CD73) produced in
E. coli is a single non-glycosylated polypeptide chain containing 247amino acids (27-252 a.a.) and having a molecular mass of 27 kDa. NT5E (CD73) is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Synonyms: NT, eN, NT5, NTE, eNT, CD73, E5NT, 5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, NT5E.
Formulation: NT5E (CD73) protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Physical Appearance: Sterile Filtered colorless solution.
Purity: Greater than 90% as determined by SDS-PAGE.
Source:E. coli.
Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MWELTILHTN DVHSRLEQTS EDSSKCVNAS RCMGGVARLF TKVQQIRRAE PNVLLLDAGD QYQGTIWFTV YKGAEVAHFM NALRYDAMAL GNHEFDNGVE GLIEPLLKEA KFPILSANIK AKGPLASQIS GLYLPYKVLP VGDEVVGIVG YTSKETPFLS NPGTNLVFED EITALQPEVD KLKTLNVNKI IALGHSGFEM DKLIAQKVRG VDVVVGGHSN TFLYTGN.
Uniprot ACC number: P21589
Transportation method: Shipped with Ice Packs.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Safety data sheet: Inquire at
info@3hbiomedical.com