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qPCR MasterMix
Catalog number: ABM-G890
Unit size: 4 x 1.25 ml
BlasTaq™ Probe 2X qPCR MasterMix is designed for TaqMan™ probe-based real-time qPCR analysis of DNA samples. The components in the MasterMix — including dNTPs, BlasTaq™ DNA polymerase, MgCl₂, and other proprietary buffer components — have been optimized to deliver superb sensitivity and performance in under one hour. BlasTaq™ is a next-generation, strategically engineered DNA polymerase that offers faster extension rates, improved processivity, and enhanced sensitivity compared to wild-type Taq polymerase. It possesses 5’-3’ polymerase activity and 5’-3’ exonuclease activity, which is essential for TaqMan probe hydrolysis during qPCR. It does not have 3’-5’ exonuclease (proofreading) activity, and produces 3’-dA-tailed amplicons, which are compatible with TA cloning if needed. This MasterMix is suitable for a variety of applications, including SNP genotyping assays, gene expression analysis, microarray validation, and high-throughput screening. ROX reference dye is provided separately, ensuring universal compatibility with most qPCR instruments. Refer to the Rox Machine Compatibility Chart for instrument-specific guidelines.
Article number:ABM-G890
Unit:pcs
Stock status:Special Order Item
Product Features:
- Designed for TaqMan Probe-based qPCR (Dye-based qPCR MasterMix also available)
- 40-70% less time than regular Taq
- 6 kb/min extension speed
- Highly robust and sensitive
- Probe-based One-Step RT-qPCR kit also available (Cat. No. G596)
- Included in abm's PCR Buffet Program
Storage Condition: Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly.
Note: BlasTaq™ Probe 2X qPCR MasterMix includes a separate vial of ROX Reference Dye.