Synonyms: TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.
Formulation: 1mg/ml in PBS (after reconstitution).
Transportation method: Shipped at Room temp
Solubility: Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
Clone: NYRhTNFa-E2.
Ig Subclass: Mouse IgG1.
Immunogen: r.Human TNF-a.
Purification Method: ion exchange.
Storage Procedures: In lyophilized form, for long periods, store at +4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C.
Type: Mouse Anti Human Monoclonal.
Applications: Direct ELISA, Western Blot, Immuneprecipitation, Intracellular staining.
Titer: In direct ELISA, using alkaline phosphatase goat anti-mouse Ig (Jackson Laboratories) 1:10,000 dilution will yield 0.7 O.D within 10 minutes.
Note: This antibody will bind very well to protein A in a buffer (PBS) containing high salt concentration (3M Nacl).
Safety data sheet: Inquire at info@3hbiomedical.com