Synonyms: HSPB1, CMT2F, HMN2B, HSP27, HSP28, HSP25, Heat shock protein beta-1, Heat shock 27 kDa protein, Stress-responsive protein 27, SRP27, HS.76067, DKFZp586P1322.
Formulation: 1mg/ml containing PBS, pH 7.4, & 0.1% Sodium Azide.
Physical Appearance: Sterile Filtered colorless solution.
Transportation method: Shipped with Ice Packs
Clone: P2A5AT.
Ig Subclass: Mouse IgG1 heavy chain and κ light chain.
Immunogen: Anti-human HSP27 mAb, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human HSP27 amino acids 1-205 purified from
E. coli.
Purification Method: HSP27 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Stability/Shelf Life: 12 months at -20°C. 1 month at 4°C.
Storage Procedures: For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles.
Type: Mouse Anti Human Monoclonal.
Applications: HSP27 antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:1,000~1:2,000 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:50.
Safety data sheet: Inquire at info@3hbiomedical.com