Formulation: 1mg/ml in PBS (after reconstitution).
Transportation method: Shipped with Ice Packs
Solubility: Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
Clone: YR-BrdU.
Ig Subclass: mouse IgG1
Immunogen: 5-iodouridine covalently coupled to KLH.
Purification Method: Protein A column
Storage Procedures: In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C.
Type: Mouse Antibody Monoclonal.
Applications: Immunohistochemistry, flow cytometry.
Titer: 1:500- 1:2,000 (assay dependent)
Note: This antibody was produced in BALB/c mice. This antibody can be used to detect proliferating cells in living tissues after incubation with BrdU which will be incorporated into newly synthesized DNA of replicating cells (during the S phase of the cell cycle), in place of thymidine during DNA replication. Antibody binding requires prior denaturation of the DNA, by exposing the cells to acid or heat.
Safety data sheet: Inquire at info@3hbiomedical.com