SARS-CoV-2 RT-qPCR Detection Kit (SCVPD) is designed to detect the presence of SARS-CoV-2 Coronavirus in respiratory specimens and serum samples following CDC guidelines (see Appendix 1). One-step TaqProbe RT-qPCR master mix is conveniently included in the kit. Please read carefully and follow these guidelines for biosafety precautions when dealing with possible infectious samples and for obtaining quality data. Included in the kit are two primer/probe sets (Cat #7038-N1 and 7038-N2) that target coronavirus SARS-CoV-2 nucleocapsid (N) gene, and the Human RPP30 gene primer/probe set (Cat #7038-RP) that targets exon 1 of human RPP30 gene and serves as a control to assess specimen quality. In addition, a non-infectious positive control (Cat #7038-Pos) and nuclease-free water are included in the kit. The positive control (Cat #7038-Pos) consists of non-infectious viral RNA fragments spiked into human small airway epithelial cells and serves to ensure reagents and instruments are working properly. Please refer to Tables 4 and 5 for results interpretation.
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