Quality Control: Each batch of TCF/LEF GFP Reporter Vector (TL-GFP) is verified for plasmid DNA purity and sequence integrity by spectrophotometry and Sanger sequencing. Functional validation is performed by transient transfection in HEK293T cells followed by Wnt pathway activation using LiCl or Wnt-3a (LiCl 10 mM or Wnt-3a 400 ng/mL, 24h). Inducible GFP expression is confirmed by fluorescence microscopy. (Figure 1).
Product Use: TL-GFP is for research use only and is not approved for human or animal use, or application in clinical or in vitro diagnostic procedures.
Shipping and Storage: The product is shipped at room temperature. Upon receipt, store at -20°C.
References: [1] Chen, B., et al. (2009). Small molecule-mediated disruption of Wnt-dependent signaling in tissue
regeneration and cancer. Nature Chemical Biology, 5(2), 100–107.
[2] Thorne, C. A., et al. (2010). Small-molecule inhibition of Wnt signaling through activation of casein
kinase 1α. Nature Chemical Biology, 6(11), 829–836.
[3] Wesslowski. J, et al. (2020). eGFP-tagged Wnt-3a enables functional analysis of Wnt trafficking and
signaling and kinetic assessment of Wnt binding to full-length Frizzled. J Biol Chem, 295(26):8759–
8774.