Quality Control: The specificity of the nucDNA long primer set is validated by PCR and gel electrophoresis. The specificity of the nucDNA short primer set is validated by qPCR with melt curve analysis and gel electrophoresis. The nucDNA damage of Reference Damaged Human DNA is determined by qPCR relative to the Reference Non-Damaged Human DNA.
Product Use: HNDQ is for research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures. HNDQ can be conducted using genomic DNA from human cultured cells or human tissues.
Shipping and Storage: The product is shipped on dry ice. Upon receipt, store the GoldNStart TaqGreen qPCR master mix (Cat no. SCMB6018a-1) in the dark at -20°C in a manual defrost freezer, the 2X LanaRana Long Range PCR Master Mix (Cat no. SCMB6098), primers (Cat no. 9008a and Cat no. SC9008b) and the reference DNA samples (Cat no. SC9008d and no. SC9008e) at -20°C in a manual defrost freezer, and the nuclease-free H2O (Cat no. SC9008c) at +4°C. Once thawed, do NOT refreeze GoldNStart TaqGreen qPCR master mix (Cat no. SCMB6018a-1), and keep in the dark at +4°C or on ice at all times.