Quality Control: Each batch of AP-1 Dual Reporter Vector (AP1RV) undergoes rigorous quality control to optimal performance. Plasmid DNA purity is verified by spectrophotometric, key regulatory elements and luciferase genes are sequence-validated by Sanger sequencing, and functional activity is confirmed via transient transfection in HEK293T cells and ± MAPK/AP-1 pathway activation (PMA 50 nM, 6h), followed by Luciferase Assay Kit (LAK, Cat#9048) to verify expected Firefly and Renilla luminescence signals (Figure 1).
Product Use: AP1RV is for research use only and is not approved for human or animal use, or application in clinical or in vitro diagnostic procedures.
Shipping and Storage: The product is shipped at room temperature. Upon receipt, store at -20°C.
References: [1] Bernard A, et al. Genome-scale functional profiling of the mammalian AP-1 signaling pathway. Proc
Natl Acad Sci USA. 2003;100(21):12153-12158.
[2] Sathya S, et al. Forskolin and phorbol 12-myristate 13-acetate modulate AP-1 factors and cell cycle
regulators in MCF-7 cells. J Cell Biochem. 2019;120(4):5427-5439.