The Ni-IDA agarose is developed for the purification of proteins with an affinity tag of six consecutive histidine residues. The His-tag sequence binds to Ni2+ cations, which are immobilized onto a solid support using iminodiacetic acid groups (IDA). Impurities are removed and the purified His-tagged proteins can be eluted by imidazole or a reduced pH solution.
Manufacturer
ABM (Applied Biological Materials)
Product Link
G253
Storage
+2–+8°C
Shipping
Ice packs