Quality Control: Each batch of p53 RE Dual Reporter Vector (P53RV) undergoes rigorous quality control to
optimal performance. Plasmid DNA purity is verified by spectrophotometric, key regulatory elements and luciferase genes are sequence-validated by Sanger sequencing, and functional activity is confirmed via transient transfection in HEK293T cells and ± p53 pathway activation (Nutlin-3a -0.3 μM, or Cisplatin 0.5 μM, overnight), followed by Luciferase Assay Kit (LAK, Cat. no. SC9048) to verify expected Firefly and Renilla luminescence signals (Figure 1).
Product Use: P53RV is for research use only and is not approved for human or animal use, or application in
clinical or in vitro diagnostic procedures.
Shipping and Storage: The product is shipped at room temperature. Upon receipt, store at -20°C.
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binding site for p53.” Nature Genetics. 1(1):45–49.
[2] Vassilev L.T., et al. (2004) “In vivo activation of the p53 pathway by small-molecule antagonists of
MDM2.” Science. 303(5659):844–848.
[3] Menendez D, et al. (2009) “Redefining the p53 response element.” PNAS. 106 (34) 14373-14378.
[4] Lalier L, Cartron PF, Olivier C, Logé C, Bougras G, Juin P, et al. (2011) “Activation of p53 by
Nutlin‑3a induces apoptosis and cellular senescence in glioma cells and synergizes with cisplatin.”
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