GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Neural Transmission and Membrane Trafficking qPCR Array Plate Layout* (8 controls in Bold and Italic)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
A | ADRA1A | BDNF | CHRNA5 | DRD2 | GABRA2 | GABRG3 | GRIK1 | GRM3 | HTR2A | OPTN | SNAP25 | β-actin |
B | ADRA1D | BTD | CHRNA6 | DRD4 | GABRA4 | GABRQ | GRIK2 | GRM4 | HTR2C | OTC | SOD1 | GAPDH |
C | ADRA2A | CHMP2B | CHRNA7 | DRD5 | GABRA5 | GABRR2 | GRIK4 | GRM6 | HTR3A | PTS | SPR | LDHA |
D | ADRB2 | CHRM1 | CHRNE | FIG4 | GABRB1 | GCH1 | GRIK5 | GRM7 | HTR4 | QDPR | TARDBP | NONO |
E | ADRB3 | CHRM4 | COMT | FUS | GABRB3 | GH1 | GRIN2A | HTR1A | HTR7 | RAB10 | TH | PPIH |
F | AKR1B1 | CHRM5 | DBH | G6PD | GABRE | GRIA1 | GRIN2B | HTR1B | LLGL1 | SLC6A2 | TPH1 | GDC |
G | ALDH5A1 | CHRNA3 | DHFR | GABBR1 | GABRG1 | GRIA2 | GRIN2C | HTR1D | LPL | SLC6A3 | VAPB | PPC |
H | ALS2 | CHRNA4 | DRD1 | GABBR2 | GABRG2 | GRIA3 | GRM1 | HTR1F | MAOA | SLC6A4 | VCP | NTC |