GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. This product is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
GeneQuery™ Human Microglial Polarization Markers qPCR Array Plate Layout*
(8 controls in Bold and Italic)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
A | ADGRE1 | EDA | HLA-DQB1 | IL6 | PTPRC | β-actin | ADGRE1 | EDA | HLA-DQB1 | IL6 | PTPRC | β-actin |
B | AIF1 | FCGR2A | HLA-DRA | IRF8 | RUNX1 | GAPDH | AIF1 | FCGR2A | HLA-DRA | IRF8 | RUNX1 | GAPDH |
C | ARG1 | FCGR2B | HLA-DRB1 | ITGAM | SOCS3 | LDHA | ARG1 | FCGR2B | HLA-DRB1 | ITGAM | SOCS3 | LDHA |
D | CD40 | FCGR2C | IL10 | ITGAX | SPHK1 | NONO | CD40 | FCGR2C | IL10 | ITGAX | SPHK1 | NONO |
E | CD68 | FCGR3A | IL13 | MRC1 | TGFB1 | PPIH | CD68 | FCGR3A | IL13 | MRC1 | TGFB1 | PPIH |
F | CD80 | FCGR3B | IL1B | MYB | TLR3 | GDC | CD80 | FCGR3B | IL1B | MYB | TLR3 | GDC |
G | CD86 | HEXB | IL1R1 | NOS2 | TLR4 | PPC | CD86 | HEXB | IL1R1 | NOS2 | TLR4 | PPC |
H | CLEC5A | HLA-DQA1 | IL4 | PTGES | TNF | NTC | CLEC5A | HLA-DQA1 | IL4 | PTGES | TNF | NTC |
* gene selection may be updated based on new research and development