GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Melanocyte Development & Pigmentation qPCR Array Plate Layout* (8 controls in Bold and Italic)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
A | ADCY3 | CTNNB1 | GRB2 | MC1R | PRKACA | β-actin | ADCY3 | CTNNB1 | GRB2 | MC1R | PRKACA | β-actin |
B | AKT1 | EDNRB | GSK3B | MITF | QPCT | GAPDH | AKT1 | EDNRB | GSK3B | MITF | QPCT | GAPDH |
C | AKT3 | EP300 | HRAS | MLANA | RPS6KA1 | LDHA | AKT3 | EP300 | HRAS | MLANA | RPS6KA1 | LDHA |
D | BCL2 | FOXD3 | KIT | OCA2 | SOS1 | NONO | BCL2 | FOXD3 | KIT | OCA2 | SOS1 | NONO |
E | BRAF | FZD3 | KITLG | PAX3 | SOX10 | PPIH | BRAF | FZD3 | KITLG | PAX3 | SOX10 | PPIH |
F | CDH1 | FZD4 | LEF1 | PIK3CA | TRP2 | GDC | CDH1 | FZD4 | LEF1 | PIK3CA | TRP2 | GDC |
G | CREB1 | GNB2L1 | MAPK1 | PMEL | TYR | PPC | CREB1 | GNB2L1 | MAPK1 | PMEL | TYR | PPC |
H | CREBBP | GPR143 | MAPK3 | POMC | TYRP1 | NTC | CREBBP | GPR143 | MAPK3 | POMC | TYRP1 | NTC |
* gene selection may be updated based on new research and development