GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Melanocyte Cell Biology qPCR Array Plate Layout* (8 controls in Bold and Italic)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
A | ABCC2 | BHLHE41 | CMPK2 | EME1 | FRMD5 | ITPR1 | MC1R | NR4A3 | PPM1H | ROPN1 | SOX10 | β-actin |
B | ADCK1 | BMPR1B | CSPG4 | EOMES | GJB1 | KCNN2 | MCAM | OCA2 | PRDM7 | RPS6KA1 | THEM6 | GAPDH |
C | ADCY1 | BRAF | CTTNBP2 | ESR2 | GNB2L1 | KIT | MCF2 | PKN3 | PRKACA | RTP4 | TLR1 | LDHA |
D | ADCY2 | BST2 | CYTL1 | FABP7 | GPR143 | LEF1 | MCOLN2 | PKNOX2 | PRKCB | RUNX3 | TRIM6 | NONO |
E | ADCY3 | CADM3 | DNMT3A | FAM124A | HELZ2 | LRRC45 | MITF | PLA1A | QPCT | RXRG | TRP2 | PPIH |
F | ANKRD37 | CD200 | DPY19L2 | FAXC | HOXB7 | LSM11 | MLANA | PLEKHH1 | RAB20 | SCG2 | TSPAN10 | GDC |
G | ANO5 | CDH19 | EDNRB | FOXD3 | HPDL | LYPD1 | MMP8 | PMEL | RNF157 | SFMBT2 | TYR | PPC |
H | ARL9 | CDKN2A | EGFL8 | FOXRED2 | HRAS | MAP2K1 | NPM2 | POMC | RNF182 | SHC4 | TYRP1 | NTC |
* gene selection may be updated based on new research and development