ScienCell's GeneQuery™ human epithelial cell biology qPCR array kit (GQH-EPI) is designed to facilitate gene expression profiling of key genes involved in human epithelial cell biology. 88 genes are selected in this kit based on public database and literature research.
GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Epithelial Cell Biology qPCR Array Plate Layout* (8 controls in Bold and Italic)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
A | APC | CDH2 | DLL4 | ERBB2 | FZD5 | HNF1A | LGR5 | NEUROG3 | PDX1 | ROCK2 | STAT1 | β-actin |
B | APOC2 | CDX1 | EEF1B2 | FGF7 | FZD7 | HNF1B | MAX | NKX2B | PFDN2 | RPL36 | STK11 | GAPDH |
C | ATOH1 | CDX2 | EGFR | FGFR1 | FZD8 | IHH | MMP9 | NOP58 | PPARD | SHROOM3 | TARS | LDHA |
D | BLIMP1 | CGN | EIF2AK3 | FGFR2 | GATA4 | JAM1 | MSI1 | NOTCH1 | PTK6 | SKIV2L | TGFBR2 | NONO |
E | BMPR1A | CLDN1 | ELF3 | FGFR3 | GATA6 | JAM2 | MXD1 | NOTCH2 | RB1 | SNAI1 | TJP1 | PPIH |
F | CALB2 | CLDN2 | EPHB2 | FKBP4 | GFI1 | KLF4 | MYBL2 | OCLN | RBMS1 | SOX9 | VAV2 | GDC |
G | CBFA2T2 | CTNNB1 | EPHB3 | FOXA1 | GFM1 | LDLR | MYC | PAX6 | RBPJ | SPDEF | VIL2 | PPC |
H | CDH1 | DDX56 | EPRS | FOXA2 | GSN | LGR4 | NEUROD1 | PCNA | ROCK1 | SPRR2A | VIM | NTC |