GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Basal Cell Carcinoma qPCR Array Plate Layout* (8 controls in Bold and Italic)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
A | APC | CD151 | CSNK1G2 | EIF4E | FZD5 | GSK3B | LRP2 | RAB23 | TCF7L1 | WNT11 | WNT5B | β-actin |
B | APC2 | CDH1 | CSNK1G3 | FAT2 | FZD6 | HHATL | MC1R | ROR2 | TCF7L2 | WNT16 | WNT7A | GAPDH |
C | AXIN1 | CEBPA | CTNNB1 | FBXW11 | FZD7 | HHIP | NET1 | SHFM1 | TGFBR2 | WNT2 | WNT7B | LDHA |
D | AXIN2 | CSNK1A1 | DHH | FZD1 | FZD8 | IFNA1 | PARP1 | SHH | TP53 | WNT2B | WNT8A | NONO |
E | BMP2 | CSNK1A1L | DVL1 | FZD10 | FZD9 | IHH | PRKACA | SMO | TXN | WNT3 | WNT8B | PPIH |
F | BMP4 | CSNK1D | DVL2 | FZD2 | GAS1 | KLF4 | PRKACB | STAT2 | WNT1 | WNT3A | WNT9A | GDC |
G | BTRC | CSNK1E | DVL3 | FZD3 | GLI2 | LEF1 | PRKACG | SUFU | WNT10A | WNT4 | WNT9B | PPC |
H | CASP3 | CSNK1G1 | EGF | FZD4 | GLI3 | LOC400927 | PTCH1 | TCF7 | WNT10B | WNT5A | ZIC2 | NTC |
* gene selection may be updated based on new research and development