GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Astrocyte Transcription Factors qPCR Array Plate Layout* (8 controls in Bold and Italic)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
A | ARID3B | DPF3 | FLI1 | HEY1 | KLF5 | MYCN | PHF11 | PRRX1 | SORBS2 | ZBTB46 | ZNF138 | β-actin |
B | BCL6 | E2F2 | FOS | HOPX | KLF6 | NFE2L3 | JADE2 | RAPGEF5 | SOX3 | ZC3HAV1 | ZNF219 | GAPDH |
C | BHLHE40 | EBF1 | FOXG1 | ID1 | KLF9 | NFIA | JADE3 | RBM20 | SUV420H1 | ZFHX3 | ZNF385D | LDHA |
D | CBX2 | EGR1 | GLI2 | IRF9 | KNTC1 | NFIB | PKNOX2 | RCOR2 | TRIT1 | ZFP14 | ZNF431 | NONO |
E | CHD7 | EMX2 | GLIPR1 | IRX2 | LHX2 | NFIC | PLAGL2 | RUNX2 | ZBTB20 | ZFP3 | ZNF443 | PPIH |
F | CSDC2 | ETS2 | GLIS3 | IRX5 | LIN28 | NFIX | POU3F2 | SALL2 | ZBTB34 | ZFP36 | ZNF462 | GDC |
G | CXXC4 | ETV4 | GTF2F2 | JUN | LIN28B | OTX1 | PRDM16 | SALL4 | ZBTB39 | ZMYND8 | ZNF679 | PPC |
H | CXXC5 | EZH2 | HES6 | KAT6A | MBNL1 | PBX2 | PRDM8 | SMARCA2 | ZBTB4 | ZNF135 | ZNF696 | NTC |
* gene selection may be updated based on new research and development