Each GeneQueryTM plate contains following controls:
1. Five target housekeeping genes (ACTB, GAPDH, LDHA, NONO, and PPIH), which enable normalization of data.
2. The Genomic DNA (gDNA) Control (GDC) detects possible gDNA contamination in the cDNA samples. It contains a primer set targeting a non-transcribed region of the genome.
3. Positive PCR Control (PPC) tests whether samples contain inhibitors or other factors that may negatively affect gene expression results. The PPC consists of a predispensed synthetic DNA template and a primer set that can amplify it. The sequence of the DNA template is not present in the human genome, and thus tests the efficiency of the polymerase chain reaction itself.
4. The No Template Control (NTC) is strongly recommended, and can be used to monitor the DNA contamination introduced during the workflow such as reagents, tips, and the lab bench
Quality Control: All the primer sets are validated by qPCR with melt curve analysis. The PCR products are analyzed by gel electrophoresis. Single band amplification is confirmed for each set of primers.
Product Use: GQH-AST is for research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures.
Shipping and Storage: The product is shipped at ambient temperature. Upon receipt, the plate should be stored at +4°C and is good for up to 12 months. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer.