GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Astrocyte Cell Biology qPCR Array Plate Layout* (8 controls in Bold and Italic)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
A | AFF2 | CD44 | CRYAB | EPM2A | HEY1 | ITGA3 | LMO2 | NHLRC1 | POMC | SCN1B | TGFB2 | β-actin |
B | ATRX | CDKL5 | DLK1 | FMR1 | HOPX | JAG1 | LPPR4 | NOTCH1 | PRDM16 | SLC25A18 | TGFB3 | GAPDH |
C | BEND6 | CDKN2B | DNER | FOXG1 | HTT | JAK1 | MAPK10 | NOTCH3 | PRRX1 | SMAD2 | TGFBR3 | LDHA |
D | BHLHE40 | CHRNA4 | DPF3 | FXR1 | IGFBP7 | KLF5 | MAPK14 | NR2E1 | PTPRE | SMAD3 | TH | NONO |
E | BHLHE41 | CLN8 | DUSP1 | FXR2 | IL1RAPL1 | LEP | MECP2 | NUFIP1 | RBPJ | STAT2 | TM4SF1 | PPIH |
F | BMP7 | CNTF | DUSP18 | GABRG2 | IL2RA | LGALS3 | MVP | NUFIP2 | RPS27A | STAT6 | TSPAN7 | GDC |
G | CACNA1H | CNTFR | EFHC1 | GFAP | IL6 | LHX2 | NFIC | OTX1 | S100A6 | SYNC | UBE3A | PPC |
H | CASP3 | COBL | EMX2 | GLIPR1 | IRS2 | LIF | NFIX | PHF11 | SCN1A | TDRD7 | ZFP3 | NTC |
* gene selection may be updated based on new research and development