Enterokinase Cleavage Enzyme is the catalytic subunit of the native porcine holoenzyme. It is highly active and specific for cleaving fusion proteins with the recognition sequence, Asp-Asp-Asp-Asp-Lys (DDDDK), in the inter-domain linker.
Unlike other site-specific proteases that cut within the recognition sequences leaving extra amino acids in the cleaved peptide products, the Enterokinase cleaves after the C-terminal end of the lysine residue and the fragment produced from the cleavage reaction does not inherent any residues from the DDDDK recognition sequence.
EK1 is a highly pure and active endotoxin-free mammalian-enterokinase-SDSPAGE enterokinase that is produced using human cell line in serum free media.
Manufacturer
ABM (Applied Biological Materials)
Components
- Enterokinase: 100 units in 100ul
- One unit is defined as the amount of Enterokinase that is required to cleave >95% of 10μg of control protein in 1 hour at 25°C.
Special Features
- Endotoxin free
- lycosylated for improved efficiency and stability
- Rapid cleavage (95% cleavage in 1 hour)
- Highly specific with minimum non-specific cleavage (even at 20x concentration)
- Stable over broad temperature range
- His-tagged for convenient removal post reaction.
Product Link
G699
Storage
-20°C
Shipping
Ice Packs