Proteins are degraded by Proteinase K, DNA is bound to the filter in the spin column, impurities are washed away, and the purified DNA is eluted from the binding matrix of the column.
The isolated DNA is of excellent quality for PCR, restriction digestion, and other downstream applications. The kit contains all necessary tubes for the complete experiment in isolation of genomic DNA.
Key Features:
- Simple, fast and efficient
- High yield and reproducible
- No phenol chloroform extraction or ethanol precipitation required
- High capacity - up to 10 μg of DNA per column
Application: Purification of up to 10 µg genomic DNA from blood samples.
Storage Condition: All components (except Proteinase K) can be stored at room temperature. Proteinase K should be kept at +4ºC for short-term or -20ºC for long-term storage. Kit components are stable for 12 months at room temperature after received. For maximum stability, store all contents at +4ºC.