Precision is a key quality in genome editing and gene modification methods. Confirmation of successful gene editing is an important step in any set up, and could save both time and resources if experimental insufficiencies are caught early on. abm’s CRISPR Genomic Cleavage Detection Kit is designed as an easy, yet effective way to verify your genomic editing process.CRISPR-edited samples are used as a template in PCR reactions targeting your specific region of interest. A pair of primers flanking each sgRNA target site is required to detect genomic cleavage using the kit, and this can be ordered from abm (Cat. No. C336 - sgRNA PCR Primer Pair Design & Synthesis Service). The products are then denatured and re-annealed to produce mismatches within the double strand. Our detection enzyme is able to recognize such mismatches and cleaves the strands to produce band sizes that are easily distinguishable upon gel analysis. The ready-to-use CRISPR Genomic Cleavage Detection Kit conveniently contains all the necessary reagents required, including a set of control template and primers to ensure reliable results. The control template has an approximate size of 750bp after PCR amplification and of 500bp and 250bp after the enzymatic cleavage. With a short processing time, this ready-to-use assay will be a great addition to any genomic-editing toolbox.Kit Features:Ease of use with simple stepsRapid set-upStreamlined protocol suitable for high-throughput applications
ABM (Applied Biological Materials)
Store all components at -20°C.