Human Seminal Vesicle Fibroblast Lysate (HSVF Lysate) is prepared from early passage Human Seminal Vesicle Fibroblasts using a modified RIPA buffer (50 mM Tris-HCl pH 7.2, 150 mM NaCl, 1% NP-40, 1mM EDTA, 1 mM EGTA, 0.4 mM PMSF, 5 µg/ml aprotinin, 5 µg/ml leupeptin, 1 µg/ml pepstatin, 1 mM Na3VO4, 5 mM NaF).
Lysate concentration is determined by Beckman Coulter DU 800 spectrophotometer.
Lysate is diluted and stored in Storage Buffer (Tris-HCl pH 6.8, 5% glycerol, 1% SDS, 0.002% bromphenol blue, 5% beta-mercaptoethanol).
This product is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.