Each primer set included in GQM-CE qPCR kit arrives lyophilized in a 2 mL vial. All primers are designed and tested under the same parameters: (i) an optimal annealing temperature of 65°C (with 2 mM Mg2+, and no DMSO); (ii) recognition of all known target gene transcript variants; and (iii) specific amplification of only one amplicon. Each primer set has been validated by qPCR by melt curve analysis and gel electrophoresis.
Each primer set is validated by qPCR melt curve and amplification curve analyses. The PCR products are analyzed by gel electrophoresis to confirm single band amplification.