All-In-One 5X RT MasterMix with gDNA Removal is a convenient and ready-to-use formulation for first-strand cDNA synthesis. Genomic DNA contamination is a common problem for accurate RNA detection and this kit solves that problem without affecting reverse transcription and first-strand cDNA synthesis. With this kit, cDNA synthesis will be simple, reliable, and reproducible. The optimized 5X RT MasterMix contains abm’s proprietary OneScript® Plus Reverse Transcriptase, RNaseOFF Ribonuclease Inhibitor, temperature-sensitive DNase, dNTPs, and a finely-balanced ratio of Oligo (dT)s and Random Primers. The high-quality cDNA synthesized from this kit can be used for a wide range of downstream applications.
Key Features:
• Efficient digestion of ss/dsDNA
• Maximal RNA protection – no harsh extraction steps required
• One tube, one reaction – no stopper solution or multiple handling steps
• Streamlined protocol suitable for high-throughput applications
• Simple setup for any RNA template
• High reproducibility and cDNA yield with genomic DNA (gDNA) removal.