Product Use: Trypan Blue solution is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Room temperature.
Shipping: Room temperature.
Procedure: Cells should be in a single cell suspension in buffered saline before counting. Since trypan blue has a higher affinity for serum protein than for cellular proteins, suspending the cells in medium containing serum will generate a dark background. Dilute trypan blue in cell suspension and immediately count using a hemocytometer or other counting apparatus.
Caution: If handled improperly, some components of this product may present a health hazard. Take appropriate precautions when handling this product, including the wearing of protective clothing and eyewear. Dispose of properly.