Fibronectin is a 440-500 kDa glycoprotein found both as cell surface proteins and in plasma. It binds to cell membrane receptors and extracellular matrix components.
The bovine plasma fibronectin is purified from bovine plasma with affinity chromatography, serving as an ideal substrate to support cell adhesion and spreading
Fibronectin quality was assured with a NuPAGE 4-12% Bis-Tris Gel stained with Coomassiebrilliant blue.
Under reducing conditions, fibronectin appeared as a doublet of 230 and 220 kDa.
ELISAassay showed that absorbance was directly proportional to the logarithm of fibronectin concentration.
Cell adhesion assay indicated that a coating with as low at 0.1 μg/cm2 of fibronectin significantlypromoted endothelial cell adhesion compared with non-coated controls.