Mouse Embryonic Fibroblast-Conditioned Medium (MEF-cm) is a serum free medium for the feeder independent culture of human pluripotent stem cells (hPSCs), including embryonic stem cells and induced pluripotent stem cells. MEF-cm is an efficient alternative to MEF feeder cells, supporting the self-renewal of hPSC and maintaining pluripotency in the presence of basic FGF and hPSC qualified matrix (e.g., BD MatrigelTM matrix). This product is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.