Colonic Epithelial Cell Medium (CoEpiCM) is a complete medium designed for optimal growth of normal colonic epithelial cells in vitro.
It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals.
The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5 % CO2/95% air.
Components
Colonic Epithelial Cell Medium (CoEpiCM) consists of 2x 500 ml of basal medium, 2x 5 ml of Colonic Epithelial Cell Growth Supplement (CoEpiCGS, Cat. SC2952) and 2x 5 ml of penicillin/streptomycin solution (P/S, Cat. SC0503).
Note: CoEpiCGS and P/S are not pre-mixed in CoEpiCM; they must be added separately to make the complete CoEpiCM.
Storage
Store the basal medium at +4oC and the CoEpiCGS and P/S solution at -20oC. Protect from light.
Shipping
Basal medium: room temperature. Medium supplements: dry ice.
Product Use
CoEpiCM is for in vitro laboratory research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.