bFGF-std MEF-cm was prepared using high quality mitomycin C treated MEF (MEF-mt, Cat. no. SCM7570-mt) derived from E13 embryos of CF1 mice. Serum free medium was conditioned with mitomycin C treated CF1 MEFs for 24 hours. The conditioned medium was collected daily for 7 days, pooled and sterilized by filtering through a 0.22 μm filter. Recombinant human basic FGF (rhbFGF, Cat. no. 104-02) was added to the medium at a final concentration of 4 ng/mL to make
the complete medium. bFGF-std MEF-cm has been tested for its ability to support hPSC self-renewal on BD MatrigelTM matrix in vitro. The medium color of each lot may vary but does not affect performance.
Component: bFGF-std MEF-cm consists of 100 mL of the medium in two 50-mL bottles.
Product Use: bFGF-std MEF-cm is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Store bFGF-std MEF-cm at ≤-20°C in a manual defrost freezer. Thaw the medium overnight at +2-8°C in the dark. Long-term storage (>2 weeks) at +2-8°C is not recommended. Avoid repeated
freeze-thaw cycles.
Shipping: Dry ice.