Human Hepatic Stellate Cell Medium-phenol red free (SteCM-prf) is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (2%).
The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air.
The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human hepatic stellate cells in vitro.
Components
SteCM-prf consists of 500 ml of basal medium, 10 ml of fetal bovine serum (FBS, Cat. No. SC0010), 5 ml of stellate cell growth supplement (SteCGS, Cat. No. SC5352) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. SC0503).
Product Use
SteCM-prf is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Store the basal medium at +4°C, the SteCGS, the FBS and the P/S solution at -20°C. Protect from light.
Shipping
Dry ice.