Pericyte Medium-mouse (PM-m), when used with Pericyte Growth Supplement (PGS-m, Cat SC1282 ) and 10 mL FBS is a complete medium designed for optimal growth of normal mouse pericytes in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (2 %). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal mouse pericytes in vitro.
Product Use
PM-m consists of 500 ml of basal medium, 10 ml of fetal bovine serum (FBS, Cat. no. SC0010), 5 ml of Pericyte Growth Supplement-mouse (PGS-m, Cat. no. SC1282) and 5 ml of penicillin/streptomycin solution (P/S, Cat. no. SC0503). Note: FBS, PGS-mand P/S are not pre-mixed in PM-m; they must be added separately to make the complete PM-m.
Storage
Store the basal medium at 4oC and the PGS-m, FBS and P/S solutions at -20oC. Protect from light.
Shipping
Basal medium: room temperature. Supplements: dry ice.