Before using MEF-cm for hPSC culture, please add fresh recombinant human basic FGF (4 ng/mL) (rhbFGF, Cat no. 104-02) into the medium to make the complete medium. The complete MEF-cm has been tested for its ability to support hPSC self-renewal on BD MatrigelTM matrix in vitro. The medium color of each lot may vary but does not affect performance.
Components: MEF-cm consists of one 100 mL bottle of the medium.
Additional Materials Required: The recombinant human basic FGF (rhbFGF, Cat no. 104-02) should be purchased separately.
Product Use: MEF-cm is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Store MEF-cm at ≤ -20ºC in a manual defrost freezer. Thaw the medium overnight at +2-8ºC in the dark. Aliquot and store the unused portions at ≤ -20ºC. Long-term storage (˃ 2 weeks) at +2-8ºC is not recommended. Avoid repeated freeze-thaw cycles.
Shipping: Dry ice.