Iscove’s Modified Dulbecco’s Medium (IMDM)

IMDM, or Iscove’s Modified Dulbecco’s Medium, is basal medium widely used for growth of rapidly proliferating, high-density cell cultures, such as Jurkat, COS-7, and macrophage cells. Supplementation is required, most commonly with 10% fetal bovine serum (FBS) for cell culture use, and requires a 5-10% CO₂ environment to maintain physiological pH. IMDM is modified as follows: With • L-Glutamine • 25mM HEPES • Sodium Bicarbonate (3.02 g/L) • Phenol Red Special media modification is available as a Custom Media Service, please contact a technical representative at technical@abmgood.com for more details.