Ham's Nutrient Mixtures were originally developed to support the clonal growth of Chinese Hamster Ovary (CHO) cells as well as HeLa and mouse L-cells. Ham's F-12 has been used for the growth of primary rat hepatocytes and rat prostate epithelial cells. A clonal toxicity assay using CHO cells has also been reported with Ham's F-12 as the medium of choice. Ham's F-12 with 25 mM HEPES provides more effective buffering condition in the optimum pH range of 7.2-7.4. Ham's F-12 with L-glutamine and 25 mM HEPES is well suited for supporting the growth of a broad range of mammalian cell lines. This product is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.