Endothelial Cell Medium-rat (ECM-r), when used with Endothelial Cell Growth Supplement-rat (ECGS-r, Cat. no. SC1062) and 25 ml of calf serum (CS, Cat. no. SC0025-cs) is a complete medium designed for optimal growth of normal rat endothelial cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of calf serum (5%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal rat endothelial cells in vitro.
Product Use
Store the basal medium at +4°C, the ECGS-r, the FBS and the P/S solution at -20°C. Protect from light.
Storage
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Shipping
Basal medium: room temperature. Supplements: dry ice.