Colonic Epithelial Cell Medium (CoEpiCM) is a complete medium designed for optimal growth of normal human colonic epithelial cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. The medium is serum-free. It is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human colonic epithelial cells in vitro. This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.