HBF from ScienCell Research Laboratories are isolated from human bronchus tissue. HBF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HBF are characterized by immunofluorescence with antibody specific to fibronectin. HBF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium: It is recommended to use Fibroblast Medium (FM, Catalog number: SC2301) for culturing HBF in vitro.
Product Use: HBF are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping: Dry ice.
References: [1] Sabatini F, Petecchia L, Tavian M, Jodon de Villeroche V, Rossi GA,. Brouty-Boye D. (2005) "Human bronchial fibroblasts exhibit a mesenchymal stem cell phenotype and multilineage differentiating potentialities." Lab Invest 85(8): 962-71.
[2] Kuwano K, Hagimoto N, Hara N. (2001) "Molecular mechanisms of pulmonary fibrosis and current treatment." Curr Mol Med 1(5): 551-73.
[3] Hoshino M, Nakamura Y, Sim J, Isoqai S. (1998) "Bronchial subepithelial fibrosis and expression of matrix metalloproteinase-9 in asthmatic airway inflamaiton." J Allergy Clin Immunol 102(5): 783-8.