Recommended Medium: It is recommended to use Alveolar Epithelial Cell Medium (AEpiCM, Cat. no. SC3201) for culturing HPAEpiC-a in vitro.
Product Use: HPAEpiC-a are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping: Dry ice.
References: [1] Crapo JD, Young SL, Fram EK, Pinkerton KE, Barry BE, Crapo RO. (1983) “Morphometric characteristics of cells in the alveolar region of mammalian lungs.” Am. Rev. Respir. Dis. 128: S42-S46.
[2] Wright JR, Dobbs LG. (1991) “Regulation of pulmonary surfactant secretion and clearance.” Annu. Rev. Physiol. 53: 395-414.
[3] Johnson MD, Widdicombe JH, Allen L, Barbry P, Dobbs LG. (2002) “Alveolar epithelial type I cells contain transport proteins and transport sodium, supporting an active role for type I cells in regulation of lung liquid homeostasis.” Proc. Natl. Acad. Sci. USA. 99(4): 1966-1971.