Trophoblast Medium (TM) is a complete medium designed for optimal growth of normal human trophoblasts in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes growth of normal human trophoblasts in vitro. This product is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.