Human CD34 Stem Cell Lysate is prepared from purified stem cells using a modified RIPA buffer (50 mM Tris-HCl pH 7.2, 150 mM NaCl, 1% NP-40, 1mM EDTA, 1 mM EGTA, 0.4 mM PMSF, 5 μg/ml aprotinin, 5 μg/ml leupeptin, 1 μg/ml pepstatin, 1 mM Na3VO4, 5 mM NaF).
Protein concentration is determined Pierce™ BCA Protein Assay.
Product Use: HM Lysate is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Store lysate immediately at -20°C upon arrival; aliquot the lysate to avoid repeated freeze/thaw cycles.
Shipping
Dry ice.