RNA preparation
Total RNA Pooled Human PBMCs is prepared from freshly isolated human peripheral blood mononuclear cells of multi-donors.
Total RNA is purified using QC validated method and has been treated with RNase-free DNase I. and dissolved in RNase free water.
Quality Control
- The cell purity for total RNA preparation was >90 %.
- RNA quality of A260/280 ratio was in the range 1.9-2.1
- RNA Integrity number (RIN) >8, was determined using an Agilent 2100 Bioanalyzer.
- Concentration of RNA was determined using Nanodrop spectrophotometer.
- cDNA synthesis was tested by RT-PCR.
Application
- cDNA synthesis
- RT-PCR and qPCR
- RNA protection assay
- Northern blot analysis
- Purification of mRNA for library construction
- PCR-select cDNA subtraction
- Microarray.
Storage
-80˚C
Shipping
Dry-ice or in ethanol solution at room temperature.