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Human PBMC cDNA, single donor
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Human PBMC cDNA, single donor
Human cDNA, 20 reactions
cDNA prepared from human PBMC from a single donor. (3HCD10)
Article number:
3HCD10
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Description
Specification
Catalog Number
3HCD10
Product Name
Human PBMC cDNA
Description
First strand cDNA is synthesized by using total RNA of human PBMCs from single donor.
Unit Size
20 reactions
Cell Purity
>90% mononuclear cells
RNA Preparation
total RNA is prepared from purified human peripheral blood mononuclear cells using a modified guanidium method.
Synthesis of cDNA
Three micrograms of human PBMC total RNA was primed with random and oligo-dT primers and reverse-transcribed by reverse transcriptase in 20 µl final volume. The reaction was stopped by heating at 85°C for 5 minutes.
Quality Control
Quality of total RNA is certified by the RNA integrity number (RIN) >8.
The cDNA is performance-tested in a control PCR using human beta-actin primers or human GAPDH primers
Stability/Shelf life
Store at -80 degree and stable for 12 months.
Applications
Comparison of gene expression patterns between biological samples Gene mutation analysis Analysis of mRNA alternative splicing Gene cloning and target sequencing
Storage Condition
Store at -80˚C.
Shipping Condition
Dry-ice
Product Usage
For in vitro laboratory research use only. Not approval for use in humans or animals.
Manufacturer
3H Biomedical AB
Country of Origin
Sweden
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