Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair. Oncostatin-A is a member of the CXC chemokine family. Human PF4 is used for the proof of heparin-induced thrombocytopenia. Furthermore it is used as an inhibitor in the angiogenesis during tumor therapy.
Synonyms: CXCL4, PF-4, PF4, Iroplact, Oncostatin-A, SCYB4, MGC138298.
Formulation: The CXCL4 protein was lyophilized in PBS buffer pH 7.4.
Physical Appearance: Sterile Filtered white lyophilized powder.
Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source: Human Platelets.
Amino acid sequence: The sequence of the first four N-terminal amino acids was determined and was found to be Glu-Ala-Glu-Glu. ISANSGL.
Stability: Human CXCL4 although stable at +25°C 1 week, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.
Solubility: It is recommended to reconstitute the lyophilized CXCL4 in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com