Keratinocyte Medium (KM) is a complete medium designed for optimal growth of normal human dermal-derived keratinocytes in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals.
The medium is serum-free.
It is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air.
KM consists of 500 ml of basal medium, 5 ml of keratinocyte growth supplement (KGS, Cat. No. SC2152) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. SC0503).
KM is for researh use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Store the basal medium at 4°C, the KGS and the P/S solution at -20°C. Protect from light.