Serum Free Human Fibroblast Medium (FM-sf) is a complete medium designed for optimal growth of normal human fibroblasts in vitro. FM-sf is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, lipids, organic and inorganic compounds, hormones, growth factor and trace minerals to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human fibroblasts in vitro.
The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air.
FM-sf consists of 500 ml of basal medium, 5 ml of fibroblast growth supplement-serum free (FGS-sf, Cat. No. SC2362), and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. SC0503).
20 x 500 ml
Supplements are store at -20oC and basal medium store at 4°C. Protect from light.
Dye ice and ambient temperature.
The product is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.