Human Perineurial Cell Lysate (HPNC Lysate) is prepared from early passage Human Perineurial Cells using a modified RIPA buffer (50 mM Tris-HCl pH 7.2, 150 mM NaCl, 1% NP-40, 1mM EDTA, 1 mM EGTA, 0.4 mM PMSF, 5 μg/ml aprotinin, 5 μg/ml leupeptin, 1 μg/ml pepstatin, 1 mM Na3VO4, 5 mM NaF).
Lysate concentration is determined by Beckman Coulter DU 800 spectrophotometer.
Lysate is diluted and stored in Storage Buffer (Tris-HCl pH 6.8, 5% glycerol, 1% SDS, 0.002% bromphenol blue, 5% β-mercaptoethanol).
Quality Control:
The quality of the lysate is confirmed by SDS-PAGE.
Application:
Western Blotting, protein analysis
Product Use: HPNC Lysate is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Store lysate immediately at -80°C upon arrival; aliquot the lysate to avoid repeated freeze/thaw cycles.
Shipping: Dry ice.
Note: It is recommended to heat the lysate for 5 min at 95°C and put on ice before loading to a SDS-PAGE gel.