Astrocyte Medium (AM) is a complete medium qualified for optimal growth of normal human astrocytes in low serum condition in vitro.
It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (2%).
The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air.
EachAM consists of 500 ml of basal medium, 10 ml of fetal bovine serum (FBS, Cat. No. SC0010), 5 ml of astrocyte growth supplement (AGS, Cat. No. SC1852) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. SC0503).
Store the basal medium at 4°C, the AGS, the FBS and the P/S solution at -20°C. Protect from light.
Dry ice and ambient temperature
AM is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.