The tissue of the central nervous system is made up neurons and glia cells. Neurons are anatomic, functional, and trophic units of the brain. Despite great variability in size and shape, all neurons share common morphologic features as highly complex network. Neurons are dynamically polarized cells that serve as the major signaling unit of the nervous system.
The hippocampal neurons play a special role in learning and memory. Cultured hippocampal neurons are a useful model for studying neuronal phenomena such as differentiation, survival, process growth, and synaptogenesis.
Human Neurons-hippocampal (HN-h) are isolated from human hippocampus and are cryopreserved at P0.
Each vial contains 1 million cells in 1 ml volume.
HN-h are characterized by immunofluorescence with antibodies specific to neurofilament, MAP2, and β-tubulin III.
HN-h are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HN-h are guaranteed to further culture under the conditions provided.
However, HN-h are not recommended for expanding or long-term cultures since the cells do not proliferate in culture.
Recommended Medium
It is recommended to use Neuronal Medium (NM, Cat. no. SC1521) for culturing HN-h in vitro.
Product Use
HN-h are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice.