Human Neural Stem Cells are differentiated from a human induced pluripotent stem cell line generated using mRNA reprogramming technology from Human Dermal Fibroblasts (HDF).
The monolayer HDF-hiPSC are efficiently converted to neural epithelium using HPSC Neural Induction Medium (PSCNIM, Cat. no. SC5931), a serum-free medium for rapid and efficient neural induction of human pluripotent stem cells (hPSCs). HDF-hiPSCs differentiate to homogenous neural stem cells within 7 days in the presence of GSK3, TGF-b, LIF factors.
The iPSC-derived Human Neural Stem Cells (iPSC-NSC) are characterized by immunofluorescence with antibodies specific to nestin and SOX2. The cell population purity: >90% of cells express Nestin and >80% of cells are SOX2 positive.
After reviving, NSC can be maintained in HPSC Neural Induction Medium as an adherent culture or form neural spheres in EGF and bFGF containing neural stem cell medium.
The cells are able to form rosette and neural tube-like structures after being passaged and plated at high density on MatrigelTM coated culture vessels in neural stem medium containing EGF and bFGF.
HiPSC-NSC can be further expanded for months as a monolayer on MatrigelTM coated culture vessels or in suspension as neural spheres when seeded on ultra-low binding culture vessels in neural stem cell medium.
NSC are multipotent and able to differentiate into various neuronal and glial subtypes. Specific patterning cues, such as SHH, retinoic acid and FGF8, can be added after reviving to direct the cells to different neural lineages.
HiPSC-NSC are cryopreserved at passage 0 and delivered frozen. Each vial contains 1 million cells in 1 ml volume.
HiPSC-NSC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.
Product Content
1 vial of frozen HiPSC-NSC (Cat. no. SC1650, 1 mL, 1 million cells/vial)
50 ml Neural Induction Medium-basal (PSCNIM, Cat. no. SC5931)
1 ml Neural Induction Medium Supplement 50x (PSCNIS, Cat. no. 5982)
Recommended Medium
It is recommended to use the provided Neural Induction Medium (PSCNIM, Cat. no. SC5931) for plating HiPSC-NSC and expanding them in the short term.
Adding ROCK inhibitor Y-27632 in the first 24 hours after reviving improves cell viability and attachment in adherent cultures. Other neural stem cell medium containing bFGF and EGF can be used for reviving and long term expanding of the cells. To form neural spheres, ultra-low binding culture vessels and neural stem cell medium containing bFGF and EGF are recommended. To differentiate the HiPSC-NSC, medium containing specific growth factors should be used.
Additional Materials Required
For adherent culture:
- BD MatrigelTM hESC-qualified matrix (BD Biosciences, Cat. no. SC354277)
- ROCK Inhibitor Y-27632 (Tocris Bioscience, Cat. no. SC1254)
For suspension culture:
- Neural stem cell medium containing EGF and bFGF
- Ultra-Low Binding Culture Plate (Cat. no. SC0383)
- ROCK Inhibitor Y-27632 (Tocris Bioscience, Cat. no. SC1254)
Product Use
iPSC-HNSC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice.